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| 129 | Testing the utility of Dynamic Susceptibility Contrast (DSC)-Perfusion toolbox for mapping the characteristics of mouse brain perfusion Shreya Jain1 and Nyoman Kurniawan1 1Centre for Advanced Imaging, The University of Queensland, Brisbane, Australia Keywords: Contrast Agents, DSC & DCE Perfusion Motivation: Perfusion studies using DSC-MRI offer insights into vascular aspects of cerebral pathophysiology by allowing us to estimate viable perfusion characteristics. Goal(s): Previous studies have shown successful implementation of DSC MRI toolbox to characterise human brain perfusion, but its application to animal data remains unexplored. Our goal is to characterize perfusion parameters in mouse model using DSC-MRI-toolbox. Approach: We slightly modified and implemented DSC-MRI-toolbox to meaure CBV, CBF and MTT in different regions of mouse brain such as cortex, hippocampus and thalumus. Results: Initial data analysis shows a successful implementation of the DSC-MRI-toolbox and allowing us to estimate perfusion parameters in preclinical data. Impact: A successful implementation of DSC-MRI toolbox in mouse models will allow us to estimate perfusion parameters such as CBV, CBF, MTT and CBV with leakage correction, which will be important for characterising tissue viability in stroke and brain injury models. |
| 3204.
| 130 | Concurrent Dual-Contrast Enhancement Using Fe3O4 Nanoparticles to Achieve a CEST Signal Controllability Feixiang Hu1, Dan Wang2, Xiaowen Ma1, Tingdan Hu1, Yali Yue3, Pu-Yeh Wu4, Wei Tang1, Tong Tong1, and Weijun Peng1 1Department of Radiology, Fudan University Shanghai Cancer Center, Shanghai, China, 2Department of Ultrasound, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai, China, 3Department of Radiology, Children’s Hospital of Fudan University, Shanghai, China, 4GE Healthcare, Beijing, China Keywords: Contrast Agents, Contrast Agent Motivation: The unique microenvironment of tumors making detection of microenvironmental alterations a valuable asset for early diagnosis of tumors. Goal(s): This study focuses on exploring impact of Fe3O4 NPs on changes in the tumor microenvironment's acidity. Approach: By combining them with CEST exogenous contrast agent, we evaluated the feasibility of dual-contrast imaging. Results: In vitro MRI results suggest that Fe3O4 NPs can act as stable reference agents under varying pH conditions, providing negative contrast for T2-weighted MRI. The CEST effect shows a strong correlation with pH, allowing for quantitative assessment of pH shifts in tumor microenvironment, and can be controlled by adjusting Fe3O4 concentration. Impact: These preliminary findings represent an initial stride toward dual-contrast imaging involving CEST and T2 contrast agents, with the potential for further expansion in future in vivo investigations. |
| 3205.
| 131 | Noninvasive Early Detection and Stage of Chronic Lung Diseases with Precision MRI (pMRI) Dongjun Li1, Zongxiang Gui1, Jingjuan Qiao1, Sophia Bamishaye1, and Jenny Yang2 1Georgia State University, Atlanta, GA, United States, 2Chemistry, Georgia State University, Atlanta, GA, United States Keywords: Contrast Agents, Lung, MRI, collagen, Protein-based contrast agent, lung fibrosis, IPF, COPD, lung cancer Motivation: MRI diagnosis of lung has a limitation due to the air in the lungs which prevents the water signals. We use precision MRI to image the small lesions or fibrosis in the lung. Goal(s): Our goal is to quantitatively stage the lung diseases by MRI contrast agents. Approach: Our novel MRI contrast agent is biomarker specific which enables it target to the lung lesions or fibrosis. Results: We are able to image the lung cancer and metastasis as well as lung fibrosis using our developed MRI contrast agent. Impact: The imaging of lung tumor lesion or fibrosis is always hindered by the lung features. Our novol contrast agents enable us to image the lung diseases at early stages. |
| 3206.
| 132 | Exploring Regional Homogeneity and Functional Connectivity Changes following Contrast Agent Injection: A Pilot Resting-State fMRI Study Li Jiang1,2, Steven Roys1,2, Rosy Linda Njonkou Tchoquess1,2, Andrew Furman1,2, Rao Gullapalli1,2, Prashant Raghavan Raghavan1, and Jiachen Zhuo1,2 1Department of Diagnostic Radiology and Nuclear Medicine, University of Maryland Baltimore, Baltimore, MD, United States, 2Center for Advanced Imaging Research, University of Maryland Baltimore, Baltimore, MD, United States Keywords: Contrast Agents, Contrast Agent, Regional Homogeneity, Functional Connectivity Motivation: Contrast agents are widely used to increase the contrast difference between normal and abnormal tissues. Existing literature primarily focuses on the structural and perfusion effects of CAs, leaving a knowledge gap regarding their potential functional repercussions. Goal(s): We aimed to explore the potential impact of CA administration on brain function. Approach: We applied a combination of regional homogeneity, ROI-to-ROI, and seed-to-voxel FC analyses to rs-fMRI data from five participants before and after CA injection. Results: Our ReHo analysis showed no significant changes of local connectivity pattern, while ROI-to-ROI and seed-to-voxel FC analyses did yield significant FC changes at network level after CA administration. Impact: Even though the small sample size, our findings may raise an intriguing possibility that CA may indeed have the potential to influence brain function, particularly when examined at a network level. |
| 3207.
| 133 | Fe(II), Co(II) and Ni(II) complexed DOTA-tetraglycinates as pH and temperature biosensors Sandeep Kumar Mishra1, ABM Zakaria1, Jelena Mihailovic1, Samuel Maritim1, Daniel Coman1,2, and Fahmeed Hyder1,2,3 1Radiology and Biomedical Imaging, Yale University, New Haven, CT, United States, 2Biomedical Engineering, Yale University, New Haven, CT, United States, 3Yale Cancer Center, Yale University, New Haven, CT, United States Keywords: Contrast Agents, Contrast Agent, pH sensor, temperature sensor, transition metal complex, MRSI, early cancer detection Motivation: Gadolinium based contrast agents (GBCAs) are reported to be toxic and associated with nephrogenic systemic fibrosis (NSF). Alternative options should be developed to minimize toxicity. Goal(s): Develop alternative biocompatible transition metal-based MRI agents for advanced imaging. Approach: Using paramagnetic high spin Fe(II), Co(II), and Ni(II) ions coordinated with well-established cyclic ligands for enhanced MRS imaging to detect cancer tumors at an early stage. Results: Fe(II)DOTA-4AmC2-, Co(II)DOTA-4AmC2-, and Ni(II)DOTA-4AmC2- were synthesized and characterized. Their in vitro pH and temperature sensing capabilities were assessed using BIRDS at 11.7T. Impact: These biocompatible pH sensors can be employed for in vivo pH mapping for early cancer diagnosis and can also be adapted for clinical applications. |
| 3208.
| 134 | Influence of Contrast-Related MRI Pulse Parameters on Signal Enhancement by Gadolinium-Based Contrast Agent in Post-Contrast MRI Sequences Eun Suk Cho1, Seung Tae Woo1, Jeong Min Choi2, Joo Hee Kim1, Jei Hee Lee1, and Jae-Joon Chung1 1Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul, Korea, Republic of, 2Ewha Womans University Seoul Hospital, Seoul, Korea, Republic of Keywords: Contrast Agents, Contrast Agent Motivation: The correlations between signal enhancement by gadolinium (Gd) complexes and pulse sequences or parameters are not well elucidated. Goal(s): To investigate the influence of repetition time (TR), echo time (TE), inversion time (TI) and flip angle (FA) on signal intensity corresponding to Gd concentration. Approach: Phantom containing various Gd concentration solutions was scanned using pulse sequences with various TR, TE, TI and FA. Results: Pulse sequences with longer TR and shorter TE increased signal intensity in lower and higher Gd concentrations, respectively. Application of longer TI and higher FA increased signal intensity at lower and higher Gd concentrations, respectively. Impact: Pulse sequences with long TR,
long TI or low FA can improve SE in human tumor tissue with Gd concentrations less
than 2 mM, while high FA is appropriate in high Gd concentration environments
such as MR angiography. |
| 3209.
| 135 | Measurement of tumor extracellular pH (pHe) by a PET/MRI co-inject reagent Xiaofei Liang1, Lorenzo Palagi2, Allysa C. Pollard3, Jorge de la Cerda1, William Schuler1, Subasinghe Appuhamillage Amali Subasinghe1, Mark D. Pagel1, and Chetan B. Dhakan1 1Department of Cancer Systems Imaging, UT MD Anderson Cancer Center, Houston, TX, United States, 2University of Turin, Turin, Italy, 3Stony Brook University, Stony Brook, NY, United States Keywords: Contrast Agents, Cancer, Simultaneous PET/MRI, 68Ga contrast agent, extracellular pH measurement, 4T1 Motivation: The measurement of extracellular pH (pHe) is very important for studying the cancer development, progression, and therapeutic response. Goal(s): Accurately measure the pHe for 4T1 tumor and 0.2 pH change in vitro independent on the concentration of MRI contrast reagent. Approach: To co-inject the synthesized pH sensitive MRI contrast reagent and the PET reagent with the same pharmacokinetic functions and DCE MRI and dynamic PET scan were obtained simultaneously. Results: The pHe change of the bicarbonate treatment for the acidosis tumor type can be measured by our PET/MRI co-inject reagent. Impact: PET/MRI co-inject reagent makes it possible for the pHe measurement independent
of the concentration of MRI contrast reagent. |
| 3210.
| 136 | Fast T1 Mapping to Assess Contrast Agent Clearing in the Human Brain Vitali Telezki1,2,3, Marlena Schnieder3, Martin Uecker2,4, Peter Dechent5, and Mathias Bähr3 1Cluster of Excellence Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells (MBExC2067), University of Göttingen, Göttingen, Germany, 2Department of Interventional and Diagnostic Radiology, University Medical Center Göttingen, Göttingen, Germany, 3Department of Neurology, University Medical Center Göttingen, Göttingen, Germany, 4Institute of Biomedical Imaging, Graz University of Technology, Graz, Austria, 5Department of Cognitive Neurology, University Medical Center Göttingen, Göttingen, Germany Keywords: Contrast Agents, Quantitative Imaging Motivation: Waste clearance mechanisms are essential to maintain homeostasis of the brain. Impairment of such mechanisms may play a key role in various diseases. Goal(s): To gain insights into waste clearance processes and propose characteristic metrics to describe them. Approach: We injected contrast agent intravenously and monitored clearance with serial T1 mapping utilizing fast single-shot T1 acquisition with radial FLASH and NLINV reconstruction in healthy volunteers. Results: Time-T1 values of full protocol with 35 acquisitions fit well to our exponential model and can be characterized by clearance time $$$\tau$$$ and biggest T1 difference max ΔT1. A dramatically reduced acquisition protocol gives similar results. Impact: Advanced fast single-shot T1 mapping is promising to characterize waste clearance function in the human brain. Because of a short whole brain acquisition time of 3 minutes it can be integrated easily into existing clinical protocols. |
| 3211.
| 137 | Biophysical models on multi-parametric maps reveal brain tissue changes in molecular environments of iron and myelin in multiple sclerosis at 3T Henri Trang1, Qianlan Chen1, Shir Filo2, Darius Mewes1,3,4, Claudia Chien1,3,5, Stefan Hetzer6, Lina Anderhalten1, Tanja Schmitz-Hübsch1,3, Alexander U. Brandt1, Aviv Mezer2, and Friedemann Paul1,3,7 1Experimental and Clinical Research Center, Max Delbrück Center for Molecular Medicine and Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, Germany, 2The Edmond and Lily Safra Center for Brain Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel, 3Neuroscience Clinical Research Center, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, Germany, 4Berlin Institute of Health at Charité – Universitätsmedizin Berlin, Biomedical Innovation Academy, Berlin, Germany, 5Department of Psychiatry and Neurosciences, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany, 6Berlin Center for Advanced Neuroimaging, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany, 7Department of Neurology, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, Germany Keywords: Biomarkers, Quantitative Imaging, brain, quantitative MRI, biophysical model Motivation: Quantitative MRI is attractive for investigating pathological tissue changes, yet translation towards clinical research remains challenging. Goal(s): Our goal was to explore sensitivity of a fast quantitative multi-parametric mapping protocol combined with biophysical models at 3T to multiple sclerosis subtypes. Approach: We combined quantitative maps of magnetization transfer saturation, proton density, longitudinal and transverse relaxation rates with relaxivity approaches to disentangle water and macromolecular tissue driven changes and to identify MRI biomarkers of focal and diffuse demyelination or inflammation. Results: We found significant alterations in white matter and lesions of surrogates for myelin and iron compared to controls and worsening with disease severity. Impact: A fast quantitative multi-parameter mapping
protocol combined with biophysical models based on in vivo relaxivity approaches
in multiple sclerosis at 3T is sensitive to brain tissue
alterations in patients, suggesting disease-specific
demyelination and inflammation. An association with disability suggests
disease-relevance. |
| 3212.
| 138 | Comparison of Relaxivity and Image Quality of Brand name vs Generic Ferumoxytol: phantom and in vivo results Rianne A van der Heijden1,2, Daiki Tamada1, James Rice1,3, and Scott B Reeder1,4,5,6,7 1Radiology, University of Wisconsin-Madison, Madison, WI, United States, 2Radiology and Nuclear Medicine, Erasmus University Medical Center, Rotterdam, Netherlands, 3Mechanical engineering, University of Wisconsin-Madison, Madison, WI, United States, 4Medical Physics, University of Wisconsin-Madison, Madison, WI, United States, 5Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, United States, 6Medicine, University of Wisconsin-Madison, Madison, WI, United States, 7Emergency Medicine, University of Wisconsin-Madison, Madison, WI, United States Keywords: Contrast Agents, Vessels, Vascular Motivation: Ferumoxytol is a superparamagnetic iron-oxide agent increasingly used for MR angiography. Cost can potentially be reduced by using a newly introduced generic ferumoxytol, so long as the relaxivity and image quality are similar. Goal(s): To compare the relaxivity and image quality of brand name ferumoxytol (Feraheme®, AMAG Pharmaceuticals) with generic ferumoxytol (Sandoz), and determine optimal dose and acquisition parameters. Approach: Phantom experiment and in-vivo dose accumulation with flip angle optimization, using an intra-individual cross-over design. Results: No clinically relevant differences in relaxivity and MR angiography image quality were observed between ferumoxytol agents. The optimal flip angle and dose are 20° and 5mg/kg, respectively. Impact: Brand name ferumoxytol (Feraheme®, AMAG Pharmaceuticals) and generic ferumoxytol (Sandoz) are interchangeable for MR angiography. The use of a generic agent may lead to reduction in cost. |
| 3213.
| 139 | Ferumoxytol Dose Optimization for 3D Whole-Heart Congenital Heart Disease Imaging: Comparison Across Three Dosing Regimens. Aya El Jerbi1, Tarique Hussain1, and Sanja Dzelebdzic1 1Cardiology, The University of Texas Southwestern Medical Center, Dallas, TX, United States Keywords: Contrast Agents, Contrast Mechanisms, Ferumoxytol Motivation: Ferumoxytol is becoming more widely used as a contrast agent, yet the optimum dose for use in cardiac MRI remains unknown. Goal(s): To evaluate whether reducing the contrast dose to 2 or 1mg/kg will affect the image quality and diagnostic accuracy. Approach: Forty-five patients with complex congenital heart disease had a 3D-IR-SSFP sequence performed at two different ferumoxytol doses. Twenty were in the ferumoxytol 1 vs. 2 mg/kg group, and twenty-five in the 2 vs. 3 mg/kg group. Results: There was no significant difference in the RCA image quality score, RCA length, diagnostic accuracy nor CNR with the use of the reduced dosing. Impact: Using reduced ferumoxytol dosing (1 or 2mg/kg), may lead to comparable image quality and diagnostic performance. The use of 2mg/kg lowers blood-T1 time to the extent that systolic imaging should not be precluded with the current 3D-IR Whole Heart Sequences. |
| 3214.
| 140 | Detection of low calcium channel activity in pig hearts with manganese-enhanced MRI Wibeke Nordhøy1, Magne Mørk Kleppestø2, Tryggve Holck Storås2, and Jonny Østensen3 1Department of Physics and Computational Radiology, Oslo University Hospital, OSLO, Norway, 2Department of Physics and Computational Radiology, Oslo University Hospital, Oslo, Norway, 3IC Targets AS, Oslo, Norway Keywords: Contrast Agents, Myocardium, MEMRI, Manganese, Mangafodipir, pig study, T tubuli, Ca2+ channel activity Motivation: Evaluate manganese-enhanced MRI (MEMRI) for heart failure assessment. Goal(s): Quantify the manganese uptake rate in the myocardium as an indicator of L-type calcium channel activity and T-tubule density. Approach: A high temporally-resolved dynamic SatRec sequence was used to track the Mangafodipir bolus instead of using the less precise, but higher spatially-resolved, conventional T1 mapping method that was used before and after the manganese administration. Results: Pigs have less dense T-tubule than humans leading to fewer L-type calcium channels and low Mn uptake in cardiomyocytes. Therefore, we believe that MEMRI can become a promising tool for assessing heart failure diseases in humans. Impact: Since pigs are often used in preclinical research, there are important differences to be aware of. The low Mn uptake proved their less
dense T-tubule, showing that MEMRI may be a useful tool for studying heart
failure in humans. |
| 3215.
| 141 | High Contrast MRI of the Prostate Using Divided Reverse Subtracted Inversion Recovery (drSIR) Sequences Philipp Matern1,2, David Bonekamp3, Julian Rauch1,2, Graeme M. Bydder4, and Tristan Anselm Kuder1,2 1Division of Medical Physics in Radiology, German Cancer Research Center (DKFZ), Heidelberg, Germany, 2Faculty of Physics and Astronomy, Heidelberg University, Heidelberg, Germany, 3Division of Radiology, German Cancer Research Center (DKFZ), Heidelberg, Germany, 4Department of Radiology, University of California San Diego, San Diego, CA, United States Keywords: Novel Contrast Mechanisms, Prostate, Inversion Recovery Motivation: Divided reverse Subtracted Inversion Recovery (drSIR) has shown potential for brain MRI but has not yet been applied to the prostate. Goal(s): To produce prostate images with high $$$T_1$$$ contrast and highlight the junction between the peripheral and transitional zones by using two IR-TSE images with different inversion times (TIs). Approach: In healthy volunteers, the TIs that suppressed the peripheral and transitional zones as well as the tissue junction were determined, and the corresponding images were subtracted and divided pixelwise. Results: High $$$T_1$$$ contrast between the prostate zones was achieved without contrast agents and subtle $$$T_1$$$ heterogeneities were observed. Impact: High $$$T_1$$$ contrast with obvious distinction between the peripheral and the transitional zones of the prostate was achieved without contrast agents. The approach is likely to show lesions with high contrast and may improve the detection and delineation of tumors. |
| 3216.
| 142 | Resotran - A positive direction for negative contrast: clinically approved Ferucarbotran reintroduced Johanna Günther1, Viktor Hartung2, Anne Marie Augustin2, Thomas Kampf1,3, Teresa Reichl1, Philipp Gruschwitz2, Martin A. Rückert1, Thorsten A. Bley2, Volker C. Behr1, Stefan Herz2,4, and Patrick Vogel1,5 1Experimental Physics 5 (Biophysics), Julius-Maximilians University, Würzburg, Germany, 2Department of Diagnostic and Interventional Radiology, University Hospital Würzburg, Würzburg, Germany, 3Department of Diagnostic and Interventional Neuroradiology, University Hospital Würzburg, Würzburg, Germany, 4Radiologie Augsburg Friedberg, Augsburg, Germany, 5Pure Devices GmbH, Rimpar, Germany Keywords: Hybrid & Novel Systems Technology, Magnetization transfer, Magnetic particle spectroscopy, MPS, COMPASS, contrast agents, iron oxide based Motivation: Contrast agents allow a significant improvement of MRI performance. Resotran is a new iron oxide-based and biocompatible MRI contrast agent that is now commercially available in Europe, which still needs to be characterized in more detail. Goal(s): Classification of the performance of Resotran compared to Resovist. Approach: Analyzation and characterization with various spectroscopic and imaging methods. Results: Resotran shows a comparable performance to the former liver contrast agent Resovist. Furthermore, it was shown that Resotran is feasible as a tracer for the new imaging modality magnetic particle imaging (MPI). This is an important step towards paving the way for MPI in clinical routine. Impact: In this work it is shown that the new clinically approved
contrast agent Resotran shows a comparable performance as the well-known
contrast agent Resovist by a detailed comparison applying different
spectroscopic characterization methods. |
| 3217.
| 143 | Noninvasive Visualization of Molecular Signatures of Liver Fibrosis Progression by Collagen Targeted Protein MRI Contrast Agent Zongxiang Gui1, Dongjun Li1, Jingjuan Qiao1, Sophia Bamishaye1, and Jenny Yang2 1Georgia State University, Atlanta, GA, United States, 2Chemistry, Georgia State University, Atlanta, GA, United States Keywords: Contrast Agents, Liver, chronic diseases, fibrosis, precision MRI Motivation: Hepatic fibrosis can develop in patients with any type of chronic liver disease which increase the risk of death for an estimated 1–2% of the world’s population. Goal(s): Our goal is to develop novel MRI contrast agents for liver diseases which enable us to detect the liver diseases at early stages. Approach: We developed the novel MRI contrast agent for precision MRI. We achieved the molecular imaging on different animal models with liver diseases. Results: Our results shows that our protein based MRI contrast agent can target the small lesions and fibrotic areas in the liver. Impact: The Protein based contrast agent with specific targeting capabilities is able to stage the liver diseases accurately. |