| 08:15 | | IntroductionJunko Kikuta Juntendo University, Bunkyo-ku, Japan |
| 08:27 | 0028.
 | The Correlation between Choroid Plexus and Protein Biomarkers in the Alzheimer’s Disease Jiaxin Li1, Yueqin Hu2, Xue Feng3, Craig H. Meyer 3, and Li Zhao1
1College of Biomedical Engineering & Instrument Science, Zhejiang University, Hangzhou, China, 2Psychology, Beijing Normal University, Beijing, China, 3Biomedical Engineering, University of Virginia, Charlottesville, VA, United States Keywords: Alzheimer's Disease, Alzheimer's Disease, choroid plexus Motivation: Dysfunction of the glymphatic system is one of the possible causes of Alzheimer’s disease (AD). We hypothesize that the choroid plexus (CP), the major site of CSF secretion, is associated with the hallmarks of AD, Aβ and tau protein deposition. Goal(s): to investigate the association between CP and hallmark proteins in the AD. Approach: Based on the proposed CP segmentation pipeline, univariate regression and stepwise regression models were employed to analyse correlations between CP and AD. Results: Our work shows that the ratio between CP and parenchyma is correlated with Aβ42 and p-tau (p<0.001) and the CP volume is correlated with t-tau (p<0.001). Impact: The proposed CP segmentation pipeline provided improved sensitivity to detect the correlations between CP/parenchyma ratio and Aβ42 and p-tau. This work may indicate the choroid plexus a possbile biomarker for AD. |
| 08:39 | 0029.
 | Choroid Plexus Area and Permeability in Dementia with Lewy Bodies Ziming Xu1, Xize Gao1, Jinghuan Gan2, Zhichao Chen2, Yong Ji3, and Huijun Chen1
1Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, China, Beijing, China, 2Department of Neurology, Beijing Friendship Hospital, Capital Medical University, Beijing, China, Beijing, China, 3Department of Neurology, Tianjin Huanhu Hospital, Tianjin, China, Tianjin, China Keywords: Other Neurodegeneration, DSC & DCE Perfusion, choroid plexus; dementia with Lewy bodies Motivation: The choroid plexus has been demonstrated to play a significant role in the pathophysiology of dementia with Lewy bodies (DLB), however the imaging characteristics are not yet explored. Goal(s): Our goal was to assess choroid plexus area and permeability based on MRI in DLB patients. Approach: DLB patients were imaged to acquire choroid plexus area and permeability and compared with patients with Alzheimer’s disease and healthy controls. Results: DLB patients exhibited larger area and lower fractional plasma volume in choroid plexus than healthy controls. Additionally, they were found to be significantly associated with the mini-mental state examination score. Impact: The larger choroid plexus area and lower fractional plasma volume detected by T1-weighted MRI and DCE-MRI in dementia with Lewy bodies provide a non-invasive and quantitative metric for advancing the diagnosis and treatment of dementia with Lewy bodies. |
| 08:51 | 0030.
 | Relaxation-exchange imaging (REXI) for the measurement of trans-barrier water exchange in choroid plexus Xuetao Wu1,2, Zejun Wang3, Yu Yin4, Qingping He5, Yi-Cheng Hsu6, Rong Xue1,2, and Ruiliang Bai7
1State Key Laboratory of Brain and Cognitive Science, Beijing MRI Center for Brain Research, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China, 2University of Chinese Academy of Sciences, Beijing, China, 3Key Laboratory of Biomedical Engineering of Ministry of Education, College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou, China, 4Department of Chemistry, Zhejiang University, Hangzhou, China, 5School of Brain Science and Brain Medicine, Zhejiang University, Hangzhou, China, 6MR Collaboration, Siemens Healthcare, Shanghai, China, 7Interdisciplinary Institute of Neuroscience and Technology, Zhejiang University School of Medicine, Hangzhou, China Keywords: Neurofluids, Neurofluids, choroid plexus, Blood-CSF barrier, relaxation exchange Motivation: Scarcity of non-invasive imaging techniques of choroid plexus function hindered our knowledge of the blood-cerebrospinal fluid barrier (BCSFB). Goal(s): We aimed to measure the trans-barrier water exchange rate in choroid plexus. Approach: We developed a new imaging method and contrast mechanism, named relaxation-exchange imaging (REXI), and validated its feasibility on both phantoms and rats. Results: REXI successfully captured the changes in proton exchange rate of urea-water phantoms at varying pH. In-vivo experiments on rats showed the potential of REXI to measure the trans-barrier water exchange in choroid plexus. Impact: Given the emerging importance of neurofluids and choroid plexus, our novel MRI
method REXI provides a way to measure the trans-barrier water exchange in CP
and a potential imaging tool to evaluate CP function in future studies. |
| 09:03 | 0031.
 | Choroid plexus atrophy in premanifest synucleinopathy using 7 Tesla MRI: an underlooked mechanism for α-synuclein accumulation? Firdaus Fabrice Hannanu1, Stephan Grimaldi1,2, Kavita Singh1,3, Subhranil Koley1, Ambra Stefani1,4, Aleksandar Videnovic5,6, Guadalupe Garcia-Gomar1,7, and Marta Bianciardi1,6
1Brainstem Imaging Laboratory, Department of Radiology, Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA, United States, 2Department of Neurology and Movement Disorders, APHM, Hôpital Universitaire Timone, Marseilles, France, 3Multiscale Imaging and Integrative Biophysics Unit, LBN, National Institute on Aging, NIH, Baltimore, MD, United States, 4Department of Neurology, Medical University of Innsbruck, Innsbruck, Austria, 5Department of Neurology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, United States, 6Division of Sleep Medicine, Harvard University, Boston, MA, United States, 7Escuela Nacional de Estudios Superiores Unidad Juriquilla, Universidad Nacional Autónoma de México, Querétaro, Mexico Keywords: Parkinson's Disease, High-Field MRI, Choroid Plexus, Synucleinopathy, RBD Motivation: As opposed to Alzheimer's disease, the mechanism linking neurotoxic protein accumulation to alterations in neurofluid turnover and in neuroimmunity due to choroid plexus (ChP) changes is understudied in premanifest synucleinopathy. Goal(s): To determine changes in the structure of ChP in premanifest synucleinopathy and to generate a ChP probabilistic atlas. Approach: ChP in multi-contrast 7 Tesla images of 12 premanifest synucleinopathy and 12 sex-and-age-matched controls were evaluated in terms of volume and signal intensity. Results: Reduced ChP volume in premanifest synucleinopathy suggested ChP atrophy that may result in neurofluid dynamics and neuroimmune function impairment; a probabilistic atlas of ChP was generated. Impact: ChP atrophy observed in premanifest synucleinopathy using high-resolution multi-contrast 7-Tesla MRI suggests a potential role of ChP in pathophysiology of synucleinopathies, urging further investigation. Probabilistic ChP atlas may aid precise MRI localization in future studies of ChP in living humans. |
| 09:15 | 0032.
 | Unveiling Choroid Plexus Changes in Multiple Sclerosis using 7T MRI Zhe Sun1,2,3 and Yulin Ge1,2
1Bernard and Irene Schwartz Center for Biomedical Imaging, Department of Radiology, NYU Grossman School of Medicine, New York, NY, United States, 2Center for Advanced Imaging Innovation and Research (CAI2R), Department of Radiology, NYU Grossman School of Medicine, New York, NY, United States, 3Vilcek Institute of Graduate Biomedical Sciences, NYU Grossman School of Medicine, New York, NY, United States Keywords: Multiple Sclerosis, Multiple Sclerosis, choroid plexus, neurodegeneration, neuroinflammation Motivation: Conventional imaging limits the assessment of choroid plexus (ChP) inflammatory activity in multiple sclerosis (MS). Goal(s): Use high-resolution MRI with 0.25mm2 matrix at 7T to assess ChP changes in MS patients compared to controls and explore correlations with lesion volumes. Approach: In 14 MS patients and 9 controls, ChPs were categorized into vascular and stromal compartments using 7T T2* imaging, and vessel-to-stroma ration was compared between the two groups.
Results: The ChP's vessel-to-stroma ratio quantified by 7T T2*w MRI was lower in MS patients, correlating negatively with lesion volume. Furthermore, the age-related decline was more rapid in MS patients compared to controls. Impact: This pilot study suggests that the vessel-to-stroma ratio of ChP, as revealed by high-resolution 2D-GRE 7T T2* imaging, could potentially serve as an imaging marker for inflammatory and degenerative changes of ChP in MS patients. |
| 09:27 | 0033.
 | MRI study of enlarged choroid plexus in patients with spinocerebellar ataxia type 3. Zhiming Zhen1,2, Yonghua Huang1, Chenghao Cao1, Yanqiu Hua1, Peiling Ou1, Wei Chen3, Wei Chen1, Zhentao Zuo4, and Chen Liu1,2
1Department of Radiology, Southwest Hospital, Army Medical University (Third Military Medical University), Chongqing 400038, China., Chongqing, China, 27T Magnetic Resonance Imaging Translational Medical Center, Southwest Hospital, Army Medical University (Third Military Medical University), Chongqing 400038, China., Chongqing, China, 3MR Research Collaboration Team, Siemens Healthineers Ltd., Wuhan 430000, China, Wuhan, China, 4State Key Laboratory of Brain and Cognitive Science, Beijing MR Center for Brain Research, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100000, China, Beijing, China Keywords: Other Neurodegeneration, Brain, spinocerebellar ataxia type 3 Motivation: The role of cerebral immune and homeostatic structures of choroid plexus (CP) in SCA3 patients remains elusive. Goal(s): The objective of this study is to investigate the volumetric changes in the choroid plexus. Approach: Whole brain of SCA3 patients was imaged using a 3T MRI scanner and the volume of CP was analyzed. Results: The findings revealed that CP volume was significantly larger in the SCA3 group compared to normal control group, exhibiting a positive correlation with both the number of ATXN3 repeats and scores of motor function abnormalities. Impact: The abnormal change of CP volume may serve as a new
marker for distinguishing SCA3 patients |
| 09:39 | 0034.
 | Cerebrovascular reactivity in choroid plexus YUECEN JIN1, Monroe Turner2, Kaisha Hazel3, Yuguang Zhao2, Beini Hu1, Dema Abdelkarim2, Binu Thomas4, Bart Rypma2, Hanzhang Lu3, and Peiying Liu1
1University of Maryland, Baltimore, Baltimore, MD, United States, 2University of Texas at Dallas, Richardson, TX, United States, 3Johns Hopkins University, Baltimore, MD, United States, 4University of Texas Southwestern Medical center, Dallas, TX, United States Keywords: Neurofluids, Arterial spin labelling, Aging, Blood vessels, Neuro, Neurofluids, Perfusion, Vascular Motivation: Choroid plexus (CP) plays an important role in the production of CSF and the formation of the blood-CSF barrier, but its vascular function is unclear. Goal(s): We aim to quantify cerebrovascular reactivity (CVR) of CP in normal controls. Approach: We applied PCASL with 5%CO2 inhalation in 92 subjects and compared CVR in gray matter, white matter and CP between young and old subjects. Results: CVR of CP was significantly lower than that of gray and white matter, but showed no age-related difference. Impact: This work provides a
reference for future studies on CVR changes of CP in pathological conditions. |
| 09:51 | 0035.
 | How chronic cortical hypoperfusion affects choroid plexus in patients with moyamoya disease? Shoko Hara1,2, Junko Kikuta2, Kaito Takabayashi2, Hongkai Chen2, Koji Kamagata2, Yoji Tanaka1, Masaaki Hori2,3, Tadashi Nariai1, Shigeki Aoki2, and Taketoshi Maehara1
1Department of Neurosurgery, Tokyo Medical and Dental University, Tokyo, Japan, 2Department of Radiology, Juntendo University Graduate School of Medicine, Tokyo, Japan, 3Department of Radiology, Toho University Omori Medical Center, Tokyo, Japan Keywords: Neurofluids, Ischemia, moyamoya disease Motivation: How chronic cortical hypoperfusion affects choroid plexus, an important structure to maintain neurofluid dynamics, has rarely reported. Goal(s): To investigate changes of choroid plexus after revascularization surgery to improve chronic hypoperfusion in patients with moyamoya disease. Approach: Eighteen adult patients with moyamoya disease were evaluated with T1WI and ASL before and one year after surgery. Choroid plexus volume and cortical perfusion were compared before and one year after the surgery. Results: After the surgery, choroid plexus volume decreased (1.65 (0.55) ml vs. 1.52 (0.51) ml; P=0.014), while cortical perfusion improved (P=0.001). Impact: Choroid plexus may be hyperactivated
and proliferated when cortical hypoperfusion and decreased glymphatic system
function exist. After the revascularization surgery and restoration of cortical
perfusion and glymphatic system function, choroid plexus may shrink to the
normal function. |
| 10:03 | 0036.
 | Fast imaging of intravenous Gadolinium-based contrast agents entrance into ventricular CSF via choroid plexus in healthy subjects Yuanqi Sun1,2,3, Di Cao1,2,3, Yinghao Li1,2,3, Jay J. Pillai4,5, Adrian Paez1, Jacob M. Pogson6, Linda Knutsson1,7, Peter B. Barker1,2, Peter C.M. Van Zijl1,2,3, Arnold Bakker7,8, Bryan K Ward6, and Jun Hua1,2
1F.M. Kirby Research Center for Functional Brain Imaging, Kennedy Krieger Institute, Baltimore, MD, United States, 2Neurosection, Division of MRI Research, Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins University School of Medicine, Baltimore, MD, United States, 3Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, United States, 4Department of Neurosurgery, Johns Hopkins University School of Medicine, Baltimore, MD, United States, 5Division of Neuroradiology, Mayo Clinic College of Medicine and Science, Rochester, MN, United States, 6Department of Otolaryngology - Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, MD, United States, 7Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD, United States, 8Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, United States Keywords: Neurofluids, DSC & DCE Perfusion, lymphatic; CSF; ISF; GBCA Motivation: Intravenously administered gadolinium-based-contrast-agents (GBCAs) can enter the lateral-ventricle (LV) via choroid-plexus (CP). However, systematic investigation of GBCA accumulation in ventricular CSF via CP in healthy subjects is limited. Goal(s): To measure GBCA-induced signal changes in the LV and CSF around CP immediately and 4 hours after intravenous GBCA administration. Approach: Dynamic-susceptibility-contrast-in-the-CSF (cDSC) MRI was performed in 25 healthy subjects. Results: At ~20s post-GBCA, GBCA-induced signal changes were detected in the CSF around CP but not in the rest of LV. After 4 hours, GBCA-induced signal changes also became significant in the entire LV. GBCA-amount in the LV showed an age correlation. Impact: These results provided direct imaging evidence that intravenous GBCA can pass the BCSFB in the CP and enter ventricular CSF in healthy subjects. |